Bicycling is not allowed on any interior trails of the Lakeshore Nature Preserve including the main Picnic Point path or paved service roads. Biking is only allowed on the following designated bicycle paths:
- The Howard Temin Lakeshore Path from the Limnology Building on the east to Oxford Drive on the west in Shorewood Hills.
- The paved path that passes through the woods from the Howard Temin Lakeshore Path to the intersection of Lake Mendota Drive and Eagle Heights Drive.
Bicycle racks are available at all parking lots, at the Lakeshore Residence Halls, and at several locations along the east end of the Howard Temin Lakeshore Path.